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HMGB4 Polyclonal antibody

HMGB4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 12787-1-AP

Print datasheet

Synonyms

high mobility group box 4, HMGB4

验证数据展示

  • WB analysis of mouse testis using 12787-1-AP

    WB analysis of mouse testis using 12787-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12787-1-AP (HMGB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 12787-1-AP

    WB analysis of human testis using 12787-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12787-1-AP (HMGB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12787-1-AP targets HMGB4 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen HMGB4 fusion protein Ag3503 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name high-mobility group box 4
Synonyms high mobility group box 4, HMGB4
Calculated Molecular Weight 186 aa, 22 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC021180
Gene Symbol HMGB4
Gene ID (NCBI) 127540
RRIDAB_10638490
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WW32
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

High mobility group protein B4 (HMGB4) is a new member in the family of HMGB proteins. Unlike other HMGB-proteins, it is a mammalian specific protein that has two HMGB-boxes but lacks the acidic tail. HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain, but not in many other tissues (PMID: 18987332). It binds strongly to nuclear structures, regulates chromatin and mediates differentiation of neuronal cells (PMID: 27608812).

实验方案

Product Specific Protocols
WB protocol for HMGB4 antibody 12787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

Nuclear exosome HMGB3 secreted by nasopharyngeal carcinoma cells promotes tumour metastasis by inducing angiogenesis.

Authors - Kaiwen Zhang
View Article