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验证数据展示

  • WB analysis of HeLa using 14286-1-AP (same clone as 14286-1-PBS)

    WB analysis of HeLa using 14286-1-AP (same clone as 14286-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14286-1-AP (HLTF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14286-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 14286-1-AP (same clone as 14286-1-PBS)

    IP experiment of HeLa using 14286-1-AP (same clone as 14286-1-PBS)

    IP result of anti-HLTF (IP:14286-1-AP, 4ug; Detection:14286-1-AP 1:500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 14286-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14286-1-AP (same clone as 14286-1-PBS)

    IF Staining of HeLa using 14286-1-AP (same clone as 14286-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14286-1-AP (HLTF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14286-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 14286-1-AP (same clone as 14286-1-PBS)

    IHC staining of human placenta using 14286-1-AP (same clone as 14286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14286-1-AP (HLTF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14286-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HLTF Polyclonal antibody, PBS Only

HLTF Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14286-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

DNA-binding protein/plasminogen activator inhibitor 1 regulator, EC:2.3.2.27, Helicase-like transcription factor, HIP116, HIP116A

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14286-1-PBS targets HLTF in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5596

Product name: Recombinant human HLTF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-354 aa of BC044659

Sequence: MSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLTSDEEVDSVLFGSLRGHVVGLRYYTGVVNNNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVVPFGANNAFTMPLHMTFWGKEENRKAVSDQLKKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQLKTEFDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKADGLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 helicase-like transcription factor
别名 DNA-binding protein/plasminogen activator inhibitor 1 regulator, EC:2.3.2.27, Helicase-like transcription factor, HIP116, HIP116A
计算分子量 114 kDa
观测分子量 114 kDa
GenBank蛋白编号BC044659
基因名称 HLTF
Gene ID (NCBI) 6596
RRIDAB_2279646
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14527
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Helicase-like Transcription Factor (HLTF) is a crucial nuclear protein and member of the SWI/SNF family of chromatin-remodeling complexes, playing a dual role in transcription regulation and DNA damage repair. It functions dually as a regulator of gene expression, utilizing its ATP-dependent helicase activity to modify chromatin structure and facilitate transcription, and as a central effector in the DNA damage response pathway. HLTF is particularly crucial for promoting error-free DNA repair at stalled replication forks via a mechanism called template switching, which prevents the accumulation of mutagenic lesions. HLTF can be detected as 114 kDa.

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