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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 65693-1-MR (same clone as 65693-1-PBS)

    FC experiment of human peripheral blood leukocytes using 65693-1-MR (same clone as 65693-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human HLA-ABC (W6/32) Mouse IgG2a RecAb (65693-1-MR, Clone: W6/32) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65693-1-PBS in a different storage buffer formulation.

  • FC experiment of human peripheral blood leukocytes using 65693-1-MR (same clone as 65693-1-PBS)

    FC experiment of human peripheral blood leukocytes using 65693-1-MR (same clone as 65693-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human HLA-ABC (W6/32) Mouse IgG2a RecAb (65693-1-MR, Clone: W6/32) (red) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65693-1-PBS in a different storage buffer formulation.

Anti-Human HLA class I (HLA-ABC) (W6/32) Mouse IgG2a Recombinant Antibody, PBS Only

HLA class I (HLA-ABC) Uni-rAb® Recombinant Antibody for FC
Cat No. 65693-1-PBS

产品说明书

CloneNo. W6/32

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

HLA-A, HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HLA-A Ready-To-Use IHC Kit. HLA-A primary antibody included.

产品信息

65693-1-PBS targets HLA class I (HLA-ABC) in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

unknow

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 major histocompatibility complex, class I, A
别名 HLA-A, HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC
计算分子量 41 kDa
GenBank蛋白编号BC003069
基因名称 HLA-A
Gene ID (NCBI) 3105
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04439
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

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