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CoraLite®555-conjugated HLA class I ABC Polyclonal antibody

HLA class I ABC Polyclonal Antibody for FC, IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

Cat No : CL555-15240

Print datasheet

Synonyms

HLA A, HLAA, HLA-A, MHC class I antigen A*3

验证数据展示

  • FC experiment of HepG2 using CL555-15240

    FC experiment of HepG2 using CL555-15240

    1X10^6 HepG2 cells were surface stained with 0.4 ug CoraLite®555 Anti-Human HLA class I ABC (CL555-15240) (red), or 0.4 ug Control Antibody. Cells were not fixed .

  • IF Staining of HepG2 using CL555-15240

    IF Staining of HepG2 using CL555-15240

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®555 HLA class I ABC antibody (CL555-15240) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 15240-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-15240 targets HLA class I ABC in IF, FC applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC
Tested Reactivity human
Immunogen HLA class I ABC fusion protein Ag7370 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name major histocompatibility complex, class I, A
Synonyms HLA A, HLAA, HLA-A, MHC class I antigen A*3
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC003069
Gene Symbol HLA-A
Gene ID (NCBI) 3105
RRIDAB_2919626
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04439
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

实验方案

Product Specific Protocols
IF protocol for CL555 HLA class I ABC antibody CL555-15240Download protocol
FC protocol for CL555 HLA class I ABC antibody CL555-15240Download protocol
Standard Protocols
Click here to view our Standard Protocols