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验证数据展示

  • WB analysis of Raji using 14670-1-AP (same clone as 14670-1-PBS)

    WB analysis of Raji using 14670-1-AP (same clone as 14670-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 14670-1-AP (same clone as 14670-1-PBS)

    IHC staining of human tonsillitis using 14670-1-AP (same clone as 14670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14670-1-AP (HLA-F antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 14670-1-AP (same clone as 14670-1-PBS)

    IHC staining of human tonsillitis using 14670-1-AP (same clone as 14670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14670-1-AP (HLA-F antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

  • IP experiment of A431 using 14670-1-AP (same clone as 14670-1-PBS)

    IP experiment of A431 using 14670-1-AP (same clone as 14670-1-PBS)

    IP result of anti-HLA-F (IP:14670-1-AP, 4ug; Detection:14670-1-AP 1:3000) with A431 cells lysate 1040 ug. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

  • Featured Product
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HLA-F Polyclonal antibody, PBS Only

HLA-F Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14670-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

14670-1-PBS targets HLA-F in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6335

Product name: Recombinant human HLA-F protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-298 aa of BC062991

Sequence: AGSHSLRYFSTAVSRPGRGEPRYIAVEYVDDTQFLRFDSDAAIPRMEPREPWVEQEGPQYWEWTTGYAKANAQTDRVALRNLLRRYNQSEAGSHTLQGMNGCDMGPDGRLLRGYHQHAYDGKDYISLNEDLRSWTAADTVAQITQRFYEAEEYAEEFRTYLEGECLELLRRYLENGKETLQRADPPKAHVAHHPISDHEATLRCWALGFYPAEITLTWQRDGEEQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPQPLILRWEQS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 major histocompatibility complex, class I, F
别名 CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen
计算分子量 39 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC062991
基因名称 HLA-F
Gene ID (NCBI) 3134
RRIDAB_1519280
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30511
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

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