验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, human placenta tissue, human spleen tissue, A431 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human tonsillitis tissue, human placenta tissue, human skin cancer tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14670-1-AP targets HLA-F in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag6335 Product name: Recombinant human HLA-F protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-298 aa of BC062991 Sequence: AGSHSLRYFSTAVSRPGRGEPRYIAVEYVDDTQFLRFDSDAAIPRMEPREPWVEQEGPQYWEWTTGYAKANAQTDRVALRNLLRRYNQSEAGSHTLQGMNGCDMGPDGRLLRGYHQHAYDGKDYISLNEDLRSWTAADTVAQITQRFYEAEEYAEEFRTYLEGECLELLRRYLENGKETLQRADPPKAHVAHHPISDHEATLRCWALGFYPAEITLTWQRDGEEQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPQPLILRWEQS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | major histocompatibility complex, class I, F |
| 别名 | CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen |
| 计算分子量 | 39 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC062991 |
| 基因名称 | HLA-F |
| Gene ID (NCBI) | 3134 |
| RRID | AB_1519280 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30511 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HLA-F antibody 14670-1-AP | Download protocol |
| IP protocol for HLA-F antibody 14670-1-AP | Download protocol |
| WB protocol for HLA-F antibody 14670-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Kidney Int Upregulation of HLA-F expression by BK polyomavirus infection induces immune recognition by KIR3DS1-positive natural killer cells. | ||
Int J Cancer Alteration of HLA-F and HLA I antigen expression in the tumor is associated with survival in patients with esophageal squamous cell carcinoma. | ||
J Immunol Immune Modifications in Fetal Membranes Overlying the Cervix Precede Parturition in Humans. | ||
Int J Biol Sci Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.
| ||
Lung Cancer HLA-F expression is a prognostic factor in patients with non-small-cell lung cancer. | ||
Oncol Lett Lesion human leukocyte antigen-F expression is associated with a poor prognosis in patients with hepatocellular carcinoma. |
