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验证数据展示

  • WB analysis using 16789-1-AP

    WB analysis using 16789-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16789-1-AP (HLA-DRB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Raji using 16789-1-AP

    IF Staining of Raji using 16789-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB3 antibody (16789-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

HLA-DRB3 Polyclonal antibody

HLA-DRB3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16789-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

MHC class II antigen DRB3, HLA DRB3, HLA DR3B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IF/ICC detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16789-1-AP targets HLA-DRB3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 HLA-DRB3 fusion protein Ag10223 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 major histocompatibility complex, class II, DR beta 3
别名 MHC class II antigen DRB3, HLA DRB3, HLA DR3B
计算分子量 266 aa, 30 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC008987
基因名称 HLA-DRB3
Gene ID (NCBI) 3125
RRIDAB_3085508
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP79483
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are heterodimers consisting of an alpha (DRA) and a beta (DRB) chain. HLA-DRB3 is a beta chain of MHC class II molecule expressed on the surface of antigen presenting cells (APC) like B cells, macrophages, and dendritic cells (PMID: 30192820). It is involved in adaptive immune responses by presenting peptide antigens to CD4+ T cells (PMID: 19830726).

实验方案

Product Specific Protocols
WB protocol for HLA-DRB3 antibody 16789-1-APDownload protocol
IF protocol for HLA-DRB3 antibody 16789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols