HLA-DRA Polyclonal antibody

HLA-DRA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17221-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

MHC class II antigen DR, HLA-DRA1, HLA-DR, HLA DRB, HLA DRA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inDaudi cells, rat spleen tissue, Raji cells, Ramos cells
Positive IP detected inRaji cells
Positive IHC detected inhuman appendicitis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy HLA-DRA Ready-To-Use IHC Kit. HLA-DRA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17221-1-AP targets HLA-DRA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, rat
文献引用反应性human, rat
免疫原 HLA-DRA fusion protein Ag11044 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 major histocompatibility complex, class II, DR alpha
别名 MHC class II antigen DR, HLA-DRA1, HLA-DR, HLA DRB, HLA DRA1
计算分子量 254 aa, 29 kDa
观测分子量 32-34 kDa
GenBank蛋白编号BC032350
基因名称 HLA-DRA
Gene ID (NCBI) 3122
ENSEMBL Gene IDENSG00000204287
RRIDAB_10639510
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01903
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HLA-DRA antibody 17221-1-APDownload protocol
IHC protocol for HLA-DRA antibody 17221-1-APDownload protocol
IF protocol for HLA-DRA antibody 17221-1-APDownload protocol
IP protocol for HLA-DRA antibody 17221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.

Authors - Zhaohui Chen
ratIF

Aging Cell

Donor BMSC-derived small extracellular vesicles relieve acute rejection post-renal allograft through transmitting Loc108349490 to dendritic cells.

Authors - Zhi-Gang Wang
humanWB

Front Immunol

An Immunological Perspective: What Happened to Pregnant Women After Recovering From COVID-19?

Authors - Sijia Zhao
humanIF

Front Immunol

Functional Change of Effector Tumor-Infiltrating CCR5+CD38+HLA-DR+CD8+ T Cells in Glioma Microenvironment.

Authors - Pin-Yuan Chen
ratIHC

Mol Med

Correction of immunosuppression in aged septic rats by human ghrelin and growth hormone through the vagus nerve-dependent inhibition of TGF-β production.

Authors - Mian Zhou
humanIHC

BMC Cancer

HLA class II molecule HLA-DRA identifies immuno-hot tumors and predicts the therapeutic response to anti-PD-1 immunotherapy in NSCLC.

Authors - Jie Mei
Loading...