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验证数据展示

  • WB analysis of COLO 320 using 14902-1-AP

    WB analysis of COLO 320 using 14902-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14902-1-AP (HKR1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

HKR1 Polyclonal antibody

HKR1 Polyclonal Antibody for WB, ELISA
Cat No. 14902-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

GLI Kruppel family member HKR1, HKR1, Protein HKR1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, HEK-293 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14902-1-AP targets HKR1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 HKR1 fusion protein Ag6694 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GLI-Kruppel family member HKR1
别名 GLI Kruppel family member HKR1, HKR1, Protein HKR1
计算分子量 73 kDa, 75 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC053845
基因名称 HKR1
Gene ID (NCBI) 284459
RRIDAB_2878091
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10072
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HKR1, also named as ZNF875, belongs to the krueppel C2H2-type zinc-finger protein family. HRK1 is a modular protein that comprises a photosensory rhodopsin, a histidine kinase, a response regulator and a putative guanylyl cyclase domain. HKR1 is related to the protonation state of the Schiff base function that provides the linkage of the retinal chromophore with a Lys side chain of the protein (PMID: 25836735). HRK1 has 2 isoforms with the molecular mass of 73-75 kDa.

实验方案

Product Specific Protocols
WB protocol for HKR1 antibody 14902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols