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验证数据展示

  • WB analysis of human saliva using 67803-1-Ig (same clone as 67803-1-PBS)

    WB analysis of human saliva using 67803-1-Ig (same clone as 67803-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

  • WB analysis of rat spleen using 67803-1-Ig (same clone as 67803-1-PBS)

    WB analysis of rat spleen using 67803-1-Ig (same clone as 67803-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

  • WB analysis of Raji using 67803-1-Ig (same clone as 67803-1-PBS)

    WB analysis of Raji using 67803-1-Ig (same clone as 67803-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

  • WB analysis of Ramos using 67803-1-Ig (same clone as 67803-1-PBS)

    WB analysis of Ramos using 67803-1-Ig (same clone as 67803-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 67803-1-Ig (same clone as 67803-1-PBS)

    WB analysis of RAW 264.7 using 67803-1-Ig (same clone as 67803-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

    IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

    IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

HK3 Monoclonal antibody, PBS Only

HK3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67803-1-PBS

产品说明书

CloneNo. 3H3C7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

EC:2.7.1.1, Hexokinase 3, hexokinase 3 (white cell), Hexokinase type III, Hexokinase-3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67803-1-PBS targets HK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag30798

Product name: Recombinant human HK3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 622-923 aa of BC028129

Sequence: LLNWTKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGYEDPRCEIGLIVGTGTNACYMEELRNVAGVPGDSGRMCINMEWGAFGDDGSLAMLSTRFDASVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTSLGVLFRGQQIQRLQTRDIFKTKFLSEIESDSLALRQVRAILEDLGLPLTSDDALMVLEVCQAVSQRAAQLCGAGVAAVVEKIRENRGLEELAVSVGVDGTLYKLHPRFSSLVAATVRELAPRCVVTFLQSEDGSGKGAALVTAVACRLAQLTRV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hexokinase 3 (white cell)
别名 EC:2.7.1.1, Hexokinase 3, hexokinase 3 (white cell), Hexokinase type III, Hexokinase-3
计算分子量 923 aa, 99 kDa
观测分子量99 kDa
GenBank蛋白编号BC028129
基因名称 HK3
Gene ID (NCBI) 3101
RRIDAB_2918566
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP52790
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hexokinases phosphorylate glucose to produce glucose-6-phosphate (G6P), the first step in most glucose metabolism pathways. Hexokinase 3 also known as HK3, similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.

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