验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, Ramos cells, RAW 264.7 cells, human saliva, rat spleen tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
67803-1-Ig targets HK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag30798 Product name: Recombinant human HK3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 622-923 aa of BC028129 Sequence: LLNWTKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGYEDPRCEIGLIVGTGTNACYMEELRNVAGVPGDSGRMCINMEWGAFGDDGSLAMLSTRFDASVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTSLGVLFRGQQIQRLQTRDIFKTKFLSEIESDSLALRQVRAILEDLGLPLTSDDALMVLEVCQAVSQRAAQLCGAGVAAVVEKIRENRGLEELAVSVGVDGTLYKLHPRFSSLVAATVRELAPRCVVTFLQSEDGSGKGAALVTAVACRLAQLTRV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | hexokinase 3 (white cell) |
| 别名 | EC:2.7.1.1, Hexokinase 3, hexokinase 3 (white cell), Hexokinase type III, Hexokinase-3 |
| 计算分子量 | 923 aa, 99 kDa |
| 观测分子量 | 99 kDa |
| GenBank蛋白编号 | BC028129 |
| 基因名称 | HK3 |
| Gene ID (NCBI) | 3101 |
| RRID | AB_2918566 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P52790 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hexokinases phosphorylate glucose to produce glucose-6-phosphate (G6P), the first step in most glucose metabolism pathways. Hexokinase 3 also known as HK3, similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for HK3 antibody 67803-1-Ig | Download protocol |
| WB protocol for HK3 antibody 67803-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Identification of HK3 as a promising immunomodulatory and prognostic target in sepsis-induced acute lung injury | ||
Biochem Biophys Res Commun HK3: A potential prognostic biomarker with metastasis inhibition capabilities in hepatocellular carcinoma | ||
Eur J Histochem Reliable hexokinase 3 protein detection in human cell lines and primary tissue | ||
J Lipid Res Hexokinase 2 Promotes ISGylation of Acyl-CoA Synthetase Long-chain Family Member 4 in Sepsis-Induced Microglia Cells |
