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验证数据展示

  • ChIP experiment of HEK-293 using 27740-1-AP

    ChIP experiment of HEK-293 using 27740-1-AP

    Chromatin was prepared from HEK-293 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 18 µg of cross-linked chromatin, 5 µg of Histone H2B Antibody (27740-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

  • WB analysis of HEK-293T using 27740-1-AP

    WB analysis of HEK-293T using 27740-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 27740-1-AP

    WB analysis using 27740-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27740-1-AP (HIST3H2BB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 27740-1-AP

    IF Staining of HEK-293 using 27740-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Histone H2B antibody (27740-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Histone H2B Polyclonal antibody

Histone H2B Polyclonal Antibody for WB, IF/ICC, ChIP, ELISA
Cat No. 27740-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ChIP, ELISA

别名

H2B3B, HIST3H2BB, H2B-clustered histone 26, H2BC26, H2B.U histone 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HEK-293T cells, HEK-293 cells
Positive IF/ICC detected inHEK-293 cells
Positive ChIP detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Chromatin immunoprecipitation (ChIP)CHIP : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27740-1-AP targets Histone H2B in WB, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ChIP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26901

Product name: Recombinant human HIST3H2BB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-126 aa of BC100855

Sequence: MPDPSKSAPAPKKGSKKAVTKAQKKDGKKRKRGRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIASEASRLAHYNKRSTITSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 3, H2bb
别名 H2B3B, HIST3H2BB, H2B-clustered histone 26, H2BC26, H2B.U histone 1
观测分子量15-20 kDa
GenBank蛋白编号BC100855
基因名称 Histone H2B
Gene ID (NCBI) 128312
RRIDAB_3085992
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N257
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST3H2BB is a replication-dependent histone that is a member of the histone H2B family.

实验方案

Product Specific Protocols
WB protocol for Histone H2B antibody 27740-1-APDownload protocol
IF protocol for Histone H2B antibody 27740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols