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验证数据展示

  • WB analysis using 18093-1-AP (same clone as 18093-1-PBS)

    WB analysis using 18093-1-AP (same clone as 18093-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18093-1-AP (Histone H1.5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 18093-1-AP (same clone as 18093-1-PBS)

    IHC staining of mouse brain using 18093-1-AP (same clone as 18093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HIST1H1B antibody included
  • IHC staining of mouse brain using 18093-1-AP (same clone as 18093-1-PBS)

    IHC staining of mouse brain using 18093-1-AP (same clone as 18093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HIST1H1B antibody included
  • IF Staining of SH-SY5Y using 18093-1-AP (same clone as 18093-1-PBS)

    IF Staining of SH-SY5Y using 18093-1-AP (same clone as 18093-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Histone H1.5 antibody (18093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 18093-1-AP (same clone as 18093-1-PBS)

    IHC staining of human colon using 18093-1-AP (same clone as 18093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HIST1H1B antibody included

Histone H1.5 Polyclonal antibody, PBS Only

Histone H1.5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18093-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

HIST1H1B, histone cluster 1, H1b, Histone, H1F5, H1-5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HIST1H1B Ready-To-Use IHC Kit. HIST1H1B primary antibody included.

产品信息

18093-1-PBS targets Histone H1.5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12818

Product name: Recombinant human HIST1H1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-226 aa of BC101581

Sequence: MSETAPAETATPAPVEKSPAKKKATKKAAGAGAAKRKATGPPVSELITKAVAASKERNGLSLAALKKALAAGGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKKAGAAKAKKPAGATPKKAKKAAGAKKAVKKTPKKAKKPAAAGVKKVAKSPKKAKAAAKPKKATKSPAKPKAVKPKAAKPKAAKPKAAKPKAAKAKKAAAKKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 1, H1b
别名 HIST1H1B, histone cluster 1, H1b, Histone, H1F5, H1-5
计算分子量 226 aa, 23 kDa
观测分子量 32 kDa
GenBank蛋白编号BC101581
基因名称 HIST1H1B
Gene ID (NCBI) 3009
RRIDAB_3085553
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16401
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone H1.5, also known as Histone Cluster 1 H1 Family Member B (HIST1H1B), is a member of the Histone H1/H5 protein family. Histone H1.5 gene has been found to be frequently mutated in colorectal cancer and follicular lymphomas. HIST1H1B overexpression contributes to tumor aggressiveness. Histone H1.5 expression is upregulated in breast cancer and predicts poor prognosis(PMID: 34746019).

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