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验证数据展示

  • WB analysis using 18093-1-AP

    WB analysis using 18093-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18093-1-AP (Histone H1.5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 18093-1-AP

    IHC staining of mouse brain using 18093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIST1H1B antibody included
  • IHC staining of mouse brain using 18093-1-AP

    IHC staining of mouse brain using 18093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIST1H1B antibody included
  • IF Staining of SH-SY5Y using 18093-1-AP

    IF Staining of SH-SY5Y using 18093-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Histone H1.5 antibody (18093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon using 18093-1-AP

    IHC staining of human colon using 18093-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIST1H1B antibody included

Histone H1.5 Polyclonal antibody

Histone H1.5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18093-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

HIST1H1B, histone cluster 1, H1b, Histone, H1F5, H1-5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, LnCaP cells
Positive IHC detected inmouse brain tissue, human colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy HIST1H1B Ready-To-Use IHC Kit. HIST1H1B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18093-1-AP targets Histone H1.5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Histone H1.5 fusion protein Ag12818 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone cluster 1, H1b
别名 HIST1H1B, histone cluster 1, H1b, Histone, H1F5, H1-5
计算分子量 226 aa, 23 kDa
观测分子量 32 kDa
GenBank蛋白编号BC101581
基因名称 HIST1H1B
Gene ID (NCBI) 3009
RRIDAB_3085553
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16401
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone H1.5, also known as Histone Cluster 1 H1 Family Member B (HIST1H1B), is a member of the Histone H1/H5 protein family. Histone H1.5 gene has been found to be frequently mutated in colorectal cancer and follicular lymphomas. HIST1H1B overexpression contributes to tumor aggressiveness. Histone H1.5 expression is upregulated in breast cancer and predicts poor prognosis(PMID: 34746019).

实验方案

Product Specific Protocols
WB protocol for Histone H1.5 antibody 18093-1-APDownload protocol
IHC protocol for Histone H1.5 antibody 18093-1-APDownload protocol
IF protocol for Histone H1.5 antibody 18093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols