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验证数据展示

  • IF Staining of U2OS using CL488-68231

    IF Staining of U2OS using CL488-68231

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 HIGD1A antibody (CL488-68231, Clone: 3A8E2 ) at dilution of 1:400.

  • FC experiment of U2OS using CL488-68231

    FC experiment of U2OS using CL488-68231

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated HIGD1A Monoclonal antibody (CL488-68231, Clone:3A8E2)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated HIGD1A Monoclonal antibody

HIGD1A Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68231

产品说明书

CloneNo. 3A8E2

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

HIG1, HIG1 domain family member 1A, HIG1 domain family member 1A, mitochondrial, HSPC010, Hypoxia-inducible gene 1 protein

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 68231-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68231 targets HIGD1A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag14027

Product name: Recombinant human HIGD1A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-93 aa of BC000601

Sequence: MSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 HIG1 hypoxia inducible domain family, member 1A
别名 HIG1, HIG1 domain family member 1A, HIG1 domain family member 1A, mitochondrial, HSPC010, Hypoxia-inducible gene 1 protein
计算分子量 93 aa, 10 kDa
观测分子量10 kDa
GenBank蛋白编号BC000601
基因名称 HIGD1A
Gene ID (NCBI) 25994
RRIDAB_3672981
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y241
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (PMID:22342701). It is induced by hypoxia induction.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 HIGD1A antibody CL488-68231Download protocol
IF protocol for CL Plus 488 HIGD1A antibody CL488-68231Download protocol
Standard Protocols
Click here to view our Standard Protocols
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