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验证数据展示

  • WB analysis using 21749-1-AP (same clone as 21749-1-PBS)

    WB analysis using 21749-1-AP (same clone as 21749-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21749-1-AP (HIGD1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 21749-1-AP (same clone as 21749-1-PBS)

    IF Staining of HepG2 using 21749-1-AP (same clone as 21749-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIGD1A antibody (21749-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • FC experiment of A2780 using 21749-1-AP (same clone as 21749-1-PBS)

    FC experiment of A2780 using 21749-1-AP (same clone as 21749-1-PBS)

    1x10^6 A2780 cells were intracellularly stained with 0.25 ug HIGD1A Polyclonal antibody (21749-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 21749-1-AP (same clone as 21749-1-PBS)

    IHC staining of mouse ovary using 21749-1-AP (same clone as 21749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21749-1-AP (HIGD1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 21749-1-AP (same clone as 21749-1-PBS)

    IHC staining of mouse ovary using 21749-1-AP (same clone as 21749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21749-1-AP (HIGD1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HIGD1A Polyclonal antibody, PBS Only

HIGD1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 21749-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

HIG1, HIG1 domain family member 1A, HIG1 domain family member 1A, mitochondrial, HSPC010, Hypoxia-inducible gene 1 protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21749-1-PBS targets HIGD1A in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14027

Product name: Recombinant human HIGD1A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-93 aa of BC000601

Sequence: MSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HIG1 hypoxia inducible domain family, member 1A
别名 HIG1, HIG1 domain family member 1A, HIG1 domain family member 1A, mitochondrial, HSPC010, Hypoxia-inducible gene 1 protein
计算分子量 93 aa, 10 kDa
观测分子量 10 kDa
GenBank蛋白编号BC000601
基因名称 HIGD1A
Gene ID (NCBI) 25994
RRIDAB_10858789
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y241
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.

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