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验证数据展示

  • WB analysis of HeLa using 80933-1-RR (same clone as 80933-1-PBS)

    WB analysis of HeLa using 80933-1-RR (same clone as 80933-1-PBS)

    Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 80933-1-RR (HIF1a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • ChIP experiment of HeLa using 80933-1-RR (same clone as 80933-1-PBS)

    ChIP experiment of HeLa using 80933-1-RR (same clone as 80933-1-PBS)

    Chromatin was prepared from HeLa cells treated with cobalt chloride (100 μM, overnight). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of HIF-1 alpha (80933-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 80933-1-RR (same clone as 80933-1-PBS)

    WB analysis of HeLa using 80933-1-RR (same clone as 80933-1-PBS)

    WB result of HIF-1 alpha antibody (80933-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells. This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80933-1-RR (same clone as 80933-1-PBS)

    IF Staining of HepG2 using 80933-1-RR (same clone as 80933-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80933-1-RR (same clone as 80933-1-PBS)

    IF Staining of HeLa using 80933-1-RR (same clone as 80933-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 80933-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80933-1-RR

    Affinity and Kinetic Characterization of 80933-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80933-1-RR against Human HIF-1 alpha were performed. The affinity constant is 0.235 nM.

  • Featured Product
  • KD/KO验证
    • {{ptg:ProductFeaturesInfo}}

HIF-1 alpha Recombinant monoclonal antibody, PBS Only

HIF-1 alpha Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA, ChIP-qPCR
Cat No. 80933-1-PBS

产品说明书

CloneNo. 2K1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA, ChIP-qPCR

别名

HIF1A, 2K1, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

80933-1-PBS targets HIF-1 alpha in WB, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA, ChIP-qPCR Application Description
经测试反应性 human
免疫原

CatNo: Ag15198

Product name: Recombinant human HIF1a protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 574-799 aa of BC012527

Sequence: LRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
别名 HIF1A, 2K1, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1
计算分子量 826 aa, 93 kDa
观测分子量120 kDa
GenBank蛋白编号BC012527
基因名称 HIF1A
Gene ID (NCBI) 3091
RRIDAB_2918921
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16665
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, PMID: 7539918).