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HIBCH Polyclonal antibody

HIBCH Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14603-1-AP

Print datasheet

Synonyms

HIB CoA hydrolase, HIBCH, HIBYL COA H

验证数据展示

  • WB analysis using 14603-1-AP

    WB analysis using 14603-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14603-1-AP (HIBCH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 14603-1-AP

    IHC staining of human liver cancer using 14603-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14603-1-AP (HIBCH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIBCH antibody included
  • IHC staining of human liver cancer using 14603-1-AP

    IHC staining of human liver cancer using 14603-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14603-1-AP (HIBCH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIBCH antibody included

经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue, human liver tissue, human heart tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HIBCH Ready-To-Use IHC Kit. HIBCH primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14603-1-AP targets HIBCH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HIBCH fusion protein Ag6149 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 3-hydroxyisobutyryl-Coenzyme A hydrolase
Synonyms HIB CoA hydrolase, HIBCH, HIBYL COA H
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC067822
Gene Symbol HIBCH
Gene ID (NCBI) 26275
RRIDAB_2116843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6NVY1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).

实验方案

Product Specific Protocols
WB protocol for HIBCH antibody 14603-1-APDownload protocol
IHC protocol for HIBCH antibody 14603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Am J Hum Genet

A Common Polymorphism in HIBCH Influences Methylmalonic Acid Concentrations in Blood Independently of Cobalamin.

Authors - Anne M Molloy
View Article