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验证数据展示

  • WB analysis using 87420-1-RR (same clone as 87420-1-PBS)

    WB analysis using 87420-1-RR (same clone as 87420-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87420-1-RR (HGS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87420-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 87420-1-RR (same clone as 87420-1-PBS)

    IP experiment of mouse brain using 87420-1-RR (same clone as 87420-1-PBS)

    IP result of anti-HGS (IP:87420-1-RR, 4ug; Detection:87420-1-RR 1:3000) with mouse brain tissue lysate 1960 ug. This data was developed using the same antibody clone with 87420-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 87420-1-RR (same clone as 87420-1-PBS)

    IF Staining of HepG2 using 87420-1-RR (same clone as 87420-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HGS antibody (87420-1-RR, Clone: 252813G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87420-1-PBS in a different storage buffer formulation.

  • IF Staining of Saos-2 using 87420-1-RR (same clone as 87420-1-PBS)

    IF Staining of Saos-2 using 87420-1-RR (same clone as 87420-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using HGS antibody (87420-1-RR, Clone: 252813G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 87420-1-PBS in a different storage buffer formulation.

  • Affinity of 87420-1-RR

    Affinity of 87420-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87420-1-RR against Human HGS were performed. The affinity constant is 5.10 nM.

HGS Recombinant monoclonal antibody, PBS Only

HGS Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 87420-1-PBS

产品说明书

CloneNo. 252813G1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, Protein pp110, ZFYVE8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HGS Ready-To-Use IHC Kit. HGS primary antibody included.

产品信息

87420-1-PBS targets HGS in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0589

Product name: Recombinant human HGS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 186-387 aa of BC003565

Sequence: GQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNRKAEGKATSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERLRQKSTYTSYPKAEPMPSASSAPPASSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHAAPTNVVENPLPETDSQPIPPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hepatocyte growth factor-regulated tyrosine kinase substrate
别名 Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, Protein pp110, ZFYVE8
计算分子量 86 kDa
观测分子量 110 kDa
GenBank蛋白编号BC003565
基因名称 HGS
Gene ID (NCBI) 9146
RRIDAB_3745532
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14964
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting STAM's function, which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein Schwannomin/merlin. The growth suppression activity of schwannoma/merlin requires HGS. The binding of schwannoma/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.

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