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HEXIM2 Polyclonal antibody

HEXIM2 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 16598-1-AP

Print datasheet

Synonyms

HEXIM2, L3, Protein HEXIM2

验证数据展示

  • WB analysis of C6 using 16598-1-AP

    WB analysis of C6 using 16598-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 16598-1-AP (HEXIM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 16598-1-AP

    IHC staining of human testis using 16598-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16598-1-AP (HEXIM2 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with HEXIM2 antibody included
  • IHC staining of human testis using 16598-1-AP

    IHC staining of human testis using 16598-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16598-1-AP (HEXIM2 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HEXIM2 antibody included
  • IF Staining of A549 using 16598-1-AP

    IF Staining of A549 using 16598-1-AP

    Immunofluorescent analysis of A549 cells using 16598-1-AP (HEXIM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inC6 cells, COLO 320 cells, HEK-293 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy HEXIM2 Ready-To-Use IHC Kit. HEXIM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16598-1-AP targets HEXIM2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen HEXIM2 fusion protein Ag9754 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hexamthylene bis-acetamide inducible 2
Synonyms HEXIM2, L3, Protein HEXIM2
Calculated Molecular Weight 286 aa, 32 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC025970
Gene Symbol HEXIM2
Gene ID (NCBI) 124790
RRIDAB_2878286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MH2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HEXIM2 antibody 16598-1-APDownload protocol
IHC protocol for HEXIM2 antibody 16598-1-APDownload protocol
IF protocol for HEXIM2 antibody 16598-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols