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HERPUD2 Polyclonal antibody

HERPUD2 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 20640-1-AP

Print datasheet

Synonyms

HERPUD family member 2, HERPUD2

验证数据展示

  • WB analysis of A549 using 20640-1-AP

    WB analysis of A549 using 20640-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 20640-1-AP (HERPUD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20640-1-AP

    IHC staining of human kidney using 20640-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20640-1-AP (HERPUD2 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20640-1-AP

    IF Staining of HepG2 using 20640-1-AP

    Immunofluorescent analysis of HepG2 cells using 20640-1-AP (HERPUD2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 20640-1-AP is HERPUD2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen HERPUD2 fusion protein Ag14702 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HERPUD family member 2
Synonyms HERPUD family member 2, HERPUD2
Calculated Molecular Weight 406 aa, 45 kDa
Observed Molecular Weight 35-45 kDa
GenBank Accession NumberBC005091
Gene Symbol HERPUD2
Gene ID (NCBI) 64224
RRIDAB_2878714
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSE4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HERPUD2, also named as Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein, is a 406 amino acid protein, which contains one ubiquitin-like domain. HERPUD2 as a single-pass membrane protein could be involved in the unfolded protein response (UPR) pathway. HERPUD2 may exstis as two isoforms 406aa,45 kDa and 297aa 33 kDa (Genebank:EAW94050).

实验方案

Product Specific Protocols
WB protocol for HERPUD2 antibody 20640-1-APDownload protocol
IHC protocol for HERPUD2 antibody 20640-1-APDownload protocol
IF protocol for HERPUD2 antibody 20640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Lett

PMID: 25681670

Authors - Chiara Riganti
View Article