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验证数据展示

  • WB analysis of A549 using 20640-1-AP

    WB analysis of A549 using 20640-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 20640-1-AP (HERPUD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20640-1-AP

    IHC staining of human kidney using 20640-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20640-1-AP (HERPUD2 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20640-1-AP

    IF Staining of HepG2 using 20640-1-AP

    Immunofluorescent analysis of HepG2 cells using 20640-1-AP (HERPUD2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

HERPUD2 Polyclonal antibody

HERPUD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20640-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

HERPUD family member 2, HERPUD2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20640-1-AP targets HERPUD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag14702

Product name: Recombinant human HERPUD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC005091

Sequence: MDQSGMEIPVTLIIKAPNQKYSDQTISCFLNWTVGKLKTHLSNVYPSKPLTKDQRLVYSGRLLPDHLQLKDILRKQDEYHMVHLVCTSRTPPSSPKSSTNRESHEALTSSSNSSSDHSGSTTPSSGQETLSLAVGSSSEGLRQRTLPQAQTDQAQSHQFPYVMQGNVDNQFPGQAAPPGFPVYPAFSPLQMLWWQQMYAH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HERPUD family member 2
别名 HERPUD family member 2, HERPUD2
计算分子量 406 aa, 45 kDa
观测分子量 35-45 kDa
GenBank蛋白编号BC005091
基因名称 HERPUD2
Gene ID (NCBI) 64224
RRIDAB_2878714
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BSE4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HERPUD2, also named as Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein, is a 406 amino acid protein, which contains one ubiquitin-like domain. HERPUD2 as a single-pass membrane protein could be involved in the unfolded protein response (UPR) pathway. HERPUD2 may exstis as two isoforms 406aa,45 kDa and 297aa 33 kDa (Genebank:EAW94050).

实验方案

Product Specific Protocols
WB protocol for HERPUD2 antibody 20640-1-APDownload protocol
IHC protocol for HERPUD2 antibody 20640-1-APDownload protocol
IF protocol for HERPUD2 antibody 20640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Lett

PMID: 25681670

Authors - Chiara Riganti
View Article