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验证数据展示

  • WB analysis using 13691-1-AP (same clone as 13691-1-PBS)

    WB analysis using 13691-1-AP (same clone as 13691-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 13691-1-AP (same clone as 13691-1-PBS)

    IP experiment of HeLa using 13691-1-AP (same clone as 13691-1-PBS)

    IP result of anti-HERC4 (IP:13691-1-AP, 4ug; Detection:13691-1-AP 1:800) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13691-1-AP (same clone as 13691-1-PBS)

    IF Staining of HeLa using 13691-1-AP (same clone as 13691-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HERC4 antibody (13691-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

  • WB analysis using 13691-1-AP (same clone as 13691-1-PBS)

    WB analysis using 13691-1-AP (same clone as 13691-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

HERC4 Polyclonal antibody, PBS Only

HERC4 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 13691-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

EC:2.3.2.26, HECT domain and RCC1-like domain-containing protein 4, hect domain and RLD 4, HECT-type E3 ubiquitin transferase HERC4, KIAA1593

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® HERC4 Ready-To-Use IHC Kit. HERC4 primary antibody included.

产品信息

13691-1-PBS targets HERC4 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4566

Product name: Recombinant human HERC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 723-1049 aa of BC039600

Sequence: NIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hect domain and RLD 4
别名 EC:2.3.2.26, HECT domain and RCC1-like domain-containing protein 4, hect domain and RLD 4, HECT-type E3 ubiquitin transferase HERC4, KIAA1593
计算分子量 1049 aa, 118 kDa
观测分子量 107 kDa
GenBank蛋白编号BC039600
基因名称 HERC4
Gene ID (NCBI) 26091
RRIDAB_10596480
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5GLZ8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HERC4, also known as HECT and RLD domain containing E3 ubiquitin protein ligase 4, is a protein encoded by the HERC4 gene in humans. HERC4 is a novel member of HERC family. The mRNA of HERC4 has been found in all examined tissues, with its levels being significantly higher in the brain and testis than in the placenta and heart. (PMID: 28430527)

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