验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue, human placenta tissue, human testis tissue, mouse testis tissue, rat testis tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13691-1-AP targets HERC4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | HERC4 fusion protein Ag4566 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hect domain and RLD 4 |
| 别名 | Probable E3 ubiquitin-protein ligase HERC4, KIAA1593, HECT-type E3 ubiquitin transferase HERC4, hect domain and RLD 4, HECT domain and RCC1-like domain-containing protein 4 |
| 计算分子量 | 1049 aa, 118 kDa |
| 观测分子量 | 107 kDa |
| GenBank蛋白编号 | BC039600 |
| 基因名称 | HERC4 |
| Gene ID (NCBI) | 26091 |
| RRID | AB_10596480 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5GLZ8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HERC4, also known as HECT and RLD domain containing E3 ubiquitin protein ligase 4, is a protein encoded by the HERC4 gene in humans. HERC4 is a novel member of HERC family. The mRNA of HERC4 has been found in all examined tissues, with its levels being significantly higher in the brain and testis than in the placenta and heart. (PMID: 28430527)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HERC4 antibody 13691-1-AP | Download protocol |
| IF protocol for HERC4 antibody 13691-1-AP | Download protocol |
| IP protocol for HERC4 antibody 13691-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife SOX4 facilitates PGR protein stability and FOXO1 expression conducive for human endometrial decidualization. | ||
Cancer Biother Radiopharm METTL3 and HERC4: Elevated Expression and Impact on Hepatocellular Carcinoma Progression |
