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验证数据展示

  • IHC staining of human breast cancer using 27459-1-AP (same clone as 27459-1-PBS)

    IHC staining of human breast cancer using 27459-1-AP (same clone as 27459-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27459-1-AP (HERC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27459-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 27459-1-AP (same clone as 27459-1-PBS)

    IF Staining of U2OS using 27459-1-AP (same clone as 27459-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HERC2 antibody (27459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27459-1-PBS in a different storage buffer formulation.

  • WB analysis using 27459-1-AP (same clone as 27459-1-PBS)

    WB analysis using 27459-1-AP (same clone as 27459-1-PBS)

    HeLa cell lysates were subjected to SDS PAGE followed by western blot with 27459-1-AP (HERC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27459-1-PBS in a different storage buffer formulation.

HERC2 Polyclonal antibody, PBS Only

HERC2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27459-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27459-1-PBS targets HERC2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26501

Product name: Recombinant human LOC440248 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 169-335 aa of BC047911

Sequence: AGSKLEKHKIKKSTKQAFAGQVDLDLLRCQQLKLYILKAGRALFSHQDKLRQILSQPAVQETGSVHTDDGAVVSPDLGDMSPEGPQPPMILLQQLLASATQPSPVKAIFDKQELEAAALAVCQCLAVESTHPSSPGFEDCSSSEATTPVAVQHIRPARVKRRKQSPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hect domain and RLD 2
别名
计算分子量527 kDa
观测分子量527 kDa
GenBank蛋白编号BC047911
基因名称 HERC2
Gene ID (NCBI) 8924
RRIDAB_2918123
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95714
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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