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HERC2 Polyclonal antibody

HERC2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 27459-1-AP

Print datasheet

Synonyms

E3 ubiquitin-protein ligase HERC2, Herc2, LOC440248

验证数据展示

  • IHC staining of human breast cancer using 27459-1-AP

    IHC staining of human breast cancer using 27459-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27459-1-AP (HERC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 27459-1-AP

    IF Staining of U2OS using 27459-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HERC2 antibody (27459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis using 27459-1-AP

    WB analysis using 27459-1-AP

    HeLa cell lysates were subjected to SDS PAGE followed by western blot with 27459-1-AP (HERC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27459-1-AP targets HERC2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IP, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen HERC2 fusion protein Ag26501 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hect domain and RLD 2
Synonyms E3 ubiquitin-protein ligase HERC2, Herc2, LOC440248
Calculated Molecular Weight527 kDa
Observed Molecular Weight527 kDa
GenBank Accession NumberBC047911
Gene Symbol HERC2
Gene ID (NCBI) 8924
RRIDAB_2918123
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HERC2 antibody 27459-1-APDownload protocol
IHC protocol for HERC2 antibody 27459-1-APDownload protocol
IF protocol for HERC2 antibody 27459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,IP

Free Radic Biol Med

Cryptochrome 1 regulates ovarian granulosa cell senescence through NCOA4-mediated ferritinophagy

Authors - Jing Ma
View Article