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验证数据展示

  • IHC staining of human breast cancer using 27459-1-AP

    IHC staining of human breast cancer using 27459-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27459-1-AP (HERC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 27459-1-AP

    IF Staining of U2OS using 27459-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HERC2 antibody (27459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis using 27459-1-AP

    WB analysis using 27459-1-AP

    HeLa cell lysates were subjected to SDS PAGE followed by western blot with 27459-1-AP (HERC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

HERC2 Polyclonal antibody

HERC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27459-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

LOC440248, E3 ubiquitin-protein ligase HERC2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27459-1-AP targets HERC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human
文献引用反应性human
免疫原 HERC2 fusion protein Ag26501 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hect domain and RLD 2
别名 LOC440248, E3 ubiquitin-protein ligase HERC2
计算分子量527 kDa
观测分子量527 kDa
GenBank蛋白编号BC047911
基因名称 HERC2
Gene ID (NCBI) 8924
RRIDAB_2918123
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HERC2 antibody 27459-1-APDownload protocol
IHC protocol for HERC2 antibody 27459-1-APDownload protocol
IF protocol for HERC2 antibody 27459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma

Authors - Weiwei Dai
View Article
humanWB

Adv Sci (Weinh)

Loss of MNX1 Sensitizes Tumors to Cytotoxic T Cells by Degradation of PD-L1 mRNA

Authors - Zhengzheng Li
View Article
humanWB,IF,IP

Free Radic Biol Med

Cryptochrome 1 regulates ovarian granulosa cell senescence through NCOA4-mediated ferritinophagy

Authors - Jing Ma
View Article