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验证数据展示

  • WB analysis of Jurkat using 24700-1-AP

    WB analysis of Jurkat using 24700-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 24700-1-AP

    IP experiment of HEK-293 using 24700-1-AP

    IP result of anti-HELB (IP:24700-1-AP, 4ug; Detection:24700-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

  • IF Staining of HeLa using 24700-1-AP

    IF Staining of HeLa using 24700-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HELB antibody (24700-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

HELB Polyclonal antibody

HELB Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 24700-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

hDHB, EC:3.6.4.12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24700-1-AP targets HELB in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20335

Product name: Recombinant human HELB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC157019

Sequence: MARSSPYLRQLQGPLLPPRDLVEEDDDYLNDDVEEDEESVFIDAEELCSGGVKAGSLPGCLRVSICDENTQETCKVFGRFPITGAWWRVKVQVKPVVGSRSYQYQVQGFPSYFLQSDMSPPNQKHICALFLKECEVSSDDVNKFLTWVKEVSNYKNLNFENLRETLRTFHKETGRKDQKQPTQNGQEELFLDNEMSLPLENTIPFRNVMTALQFPKIMEFLPVLLPRHFKWIIGSGSKEMLKEIEEILGTHPWKLGFSKITYREWKLLRCEASWIAFCQCESLLQLMTDLEKNALIMYSRLKQICREDGHTYVEVNDLTLTLSNHMSFHAASESLKFLKDIGVVTYEKSC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 helicase (DNA) B
别名 hDHB, EC:3.6.4.12
计算分子量 1087 aa, 123 kDa
观测分子量 120-140 kDa
GenBank蛋白编号BC157019
基因名称 HELB
Gene ID (NCBI) 92797
RRIDAB_2879680
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NG08
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HELB antibody 24700-1-APDownload protocol
IF protocol for HELB antibody 24700-1-APDownload protocol
IP protocol for HELB antibody 24700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols