×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 32026-1-AP (same clone as 32026-1-PBS)

    WB analysis using 32026-1-AP (same clone as 32026-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32026-1-AP (HECW2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32026-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 32026-1-AP (same clone as 32026-1-PBS)

    IF Staining of A549 using 32026-1-AP (same clone as 32026-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HECW2 antibody (32026-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 32026-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 32026-1-AP (same clone as 32026-1-PBS)

    IHC staining of mouse cerebellum using 32026-1-AP (same clone as 32026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32026-1-AP (HECW2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32026-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 32026-1-AP (same clone as 32026-1-PBS)

    IHC staining of rat cerebellum using 32026-1-AP (same clone as 32026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 32026-1-AP (HECW2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32026-1-PBS in a different storage buffer formulation.

HECW2 Polyclonal antibody, PBS Only

HECW2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 32026-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

E3 ubiquitin-protein ligase HECW2, EC:2.3.2.26, HECT, C2 and WW domain-containing protein 2, HECT-type E3 ubiquitin transferase HECW2, KIAA1301

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

32026-1-PBS targets HECW2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36316

Product name: Recombinant human HECW2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 450-650 aa of XM_017004563.1

Sequence: SFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTHEAASFEDKPENLPELAESSLPAGPAPEEGEGGPEPQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSSCNESVT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
别名 E3 ubiquitin-protein ligase HECW2, EC:2.3.2.26, HECT, C2 and WW domain-containing protein 2, HECT-type E3 ubiquitin transferase HECW2, KIAA1301
计算分子量176 kDa，1572aa
观测分子量240 kDa
GenBank蛋白编号XM_017004563.1
基因名称 HECW2
Gene ID (NCBI) 57520
RRIDAB_3742511
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9P2P5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HECW2, also known as NEDL2 (Nedd4-like E3 ubiquitin-protein ligase 2), is a member of the HECT (Homologous to the E6-AP Carboxyl Terminus) family of E3 ubiquitin ligases. It is crucial in various pathophysiological processes, including cell proliferation, apoptosis, autophagy, immune response, and DNA damage repair (PMID: 39529070). HECW2 is known to stabilize and enhance the transcriptional activity of p73, a member of the p53 family of tumor suppressors, which promotes cell cycle arrest and apoptosis and has an essential role in neurodevelopment (PMID: 27389779). In cancer, HECW2 is a promising target for tumor therapy due to its role in regulating cell cycle progression and angiogenesis (PMID: 39529070). In neurodevelopmental disorders, variants in HECW2 have been reported to cause a disorder characterized by hypotonia, seizures, and impaired language (PMID: 34321324).

||
New chat

Able™

正在加载，请稍候...