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验证数据展示

  • WB analysis using 83859-1-RR

    WB analysis using 83859-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83859-1-RR (HEC1/HEC/NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83859-1-RR

    FC experiment of HeLa using 83859-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEC1/HEC/NDC80 Recombinant antibody (83859-1-RR, Clone:5A2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 83859-1-RR

    IF Staining of HeLa using 83859-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

HEC1/HEC/NDC80 Recombinant antibody

HEC1/HEC/NDC80 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83859-1-RR

产品说明书

CloneNo. 5A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

NDC80, Kinetochore protein Hec1, hsNDC80, HsHec1, HEC1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, Jurkat cells, NCCIT cells, K-562 cells, HEK-293T cells, A431 cells, MCF-7 cells, HepG2 cells, MOLT-4 cells
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83859-1-RR targets HEC1/HEC/NDC80 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NDC80 homolog, kinetochore complex component (S. cerevisiae)
别名 NDC80, Kinetochore protein Hec1, hsNDC80, HsHec1, HEC1
观测分子量 74 kDa
GenBank蛋白编号/
基因名称 NDC80
Gene ID (NCBI) 10403
RRIDAB_3671443
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDC80 complex is a heterotetrameric protein complex comprised of Hec1 (also called Ndc80), Nuf2, Spc24, and Spc25. Mis12 complex (bound to CENP-C or CENP-T) or CDK1-phosphorylated CENP-T to anchor the NDC80 complex to the kinetochore. HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.

实验方案

Product Specific Protocols
WB protocol for HEC1/HEC/NDC80 antibody 83859-1-RRDownload protocol
IF protocol for HEC1/HEC/NDC80 antibody 83859-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols