验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, U-251 cells, RT-4 cells |
Planning an IHC experiment? We recommend our IHCeasy® HEATR5B Ready-To-Use IHC Kit. HEATR5B primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32316-1-AP targets HEATR5B in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36352 Product name: Recombinant human HEATR5B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 700-850 aa of BC150605 Sequence: FTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | HEAT repeat containing 5B |
| 别名 | HEAT repeat-containing protein 5B, KIAA1414, p200, p200a |
| 观测分子量 | ~210 kDa |
| GenBank蛋白编号 | BC150605 |
| 基因名称 | HEATR5B |
| Gene ID (NCBI) | 54497 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9P2D3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HEATR5B (HEAT Repeat Containing 5B) is a protein containing HEAT repeat that binds directly to the dynein tail and dynactin and has an evolutionarily conserved function in promoting the motility of adaptor protein-1 (AP1)-associated endosomal membranes (PMID: 37872872).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HEATR5B antibody 32316-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
