×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of HeLa using 60064-2-Ig

    WB analysis of HeLa using 60064-2-Ig

    WB result of HDGF antibody (60064-2-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDGF transfected HeLa cells.

  • WB analysis using 60064-2-Ig

    WB analysis using 60064-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60064-2-Ig (HDGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60064-2-Ig

    WB analysis using 60064-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60064-2-Ig (HDGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • KD/KO Validated

HDGF Monoclonal antibody

HDGF Monoclonal Antibody for WB, ELISA
Cat No. 60064-2-Ig

产品说明书

CloneNo. 1A10D5

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, ELISA

别名

Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG-1L2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHT-29 cells, HeLa cells, Caki-1 cells, SCaBER cells, MG-63 cells, MCF-10A cells, MCF-7 cells, MOLT-4 cells, Karpas-299 cells
Planning an IHC experiment? We recommend our IHCeasy HDGF Ready-To-Use IHC Kit. HDGF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60064-2-Ig targets HDGF in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1893

Product name: Recombinant human HDGF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-240 aa of BC018991

Sequence: MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 hepatoma-derived growth factor (high-mobility group protein 1-like)
别名 Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG-1L2
计算分子量 26 kDa
观测分子量40 kDa
GenBank蛋白编号BC018991
基因名称 HDGF
Gene ID (NCBI) 3068
RRIDAB_3743210
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP51858
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)

实验方案

Product Specific Protocols
WB protocol for HDGF antibody 60064-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...