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HDAC3 Recombinant antibody

HDAC3 Recombinant Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2N1

Cat No : 81211-1-RR

Print datasheet

Synonyms

HD3, HDAC3, histone deacetylase 3, RPD3, RPD3 2, SMAP45

验证数据展示

  • WB analysis using 81211-1-RR

    WB analysis using 81211-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81211-1-RR (HDAC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 81211-1-RR

    IHC staining of human liver cancer using 81211-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC3 antibody included
  • IHC staining of human liver cancer using 81211-1-RR

    IHC staining of human liver cancer using 81211-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC3 antibody included
  • IHC staining of human thyroid cancer using 81211-1-RR

    IHC staining of human thyroid cancer using 81211-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC3 antibody included
  • IHC staining of human thyroid cancer using 81211-1-RR

    IHC staining of human thyroid cancer using 81211-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC3 antibody included

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MOLT-4 cells, Caco-2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman thyroid cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HDAC3 Ready-To-Use IHC Kit. HDAC3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81211-1-RR targets HDAC3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen HDAC3 fusion protein Ag0396 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone deacetylase 3
Synonyms HD3, HDAC3, histone deacetylase 3, RPD3, RPD3 2, SMAP45
Calculated Molecular Weight 49 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC000614
Gene Symbol HDAC3
Gene ID (NCBI) 8841
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15379
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (-amino groups of lysines located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene.

实验方案

Product Specific Protocols
WB protocol for HDAC3 antibody 81211-1-RRDownload protocol
IHC protocol for HDAC3 antibody 81211-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols