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Species Reactivity
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0
Conjugate
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验证数据展示

  • WB analysis using 81211-1-RR (same clone as 81211-1-PBS)

    WB analysis using 81211-1-RR (same clone as 81211-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81211-1-RR (HDAC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

  • WB analysis of HAP1 using 81211-1-RR (same clone as 81211-1-PBS)

    WB analysis of HAP1 using 81211-1-RR (same clone as 81211-1-PBS)

    WB result of HDAC3 antibody (81211-1-RR; 1:2000; room temperature for 1.5 hours) with wild-type and sg-HDAC3 transfected HAP1 cells This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

    IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC3 antibody included
  • IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

    IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC3 antibody included
  • IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

    IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC3 antibody included
  • IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

    IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC3 antibody included
  • Affinity and Kinetic Characterization of 81211-1-RR

    Affinity and Kinetic Characterization of 81211-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81211-1-RR against Human HDAC3 were performed. The affinity constant is 0.213 nM.

  • Featured Product
  • KD/KO验证
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HDAC3 Recombinant monoclonal antibody, PBS Only

HDAC3 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81211-1-PBS

产品说明书

CloneNo. 2N1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

2N1, EC:3.5.1.-, EC:3.5.1.98, HDAC 3, Histone deacetylase 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HDAC3 Ready-To-Use IHC Kit. HDAC3 primary antibody included.

产品信息

81211-1-PBS targets HDAC3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0396

Product name: Recombinant human HDAC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 187-395 aa of BC000614

Sequence: VMTVSFHKYGNYFFPGTGDMYEVGAESGRYYCLNVPLRDGIDDQSYKHLFQPVINQVVDFYQPTCIVLQCGADSLGCDRLGCFNLSIRGHGECVEYVKSFNIPLLVLGGGGYTVRNVARCWTYETSLLVEEAISEELPYSEYFEYFAPDFTLHPDVSTRIENQNSRQYLDQIRQTIFENLKMLNHAPSVQIHDVPADLLTYDRTDEADA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone deacetylase 3
别名 2N1, EC:3.5.1.-, EC:3.5.1.98, HDAC 3, Histone deacetylase 3
计算分子量 49 kDa
观测分子量49 kDa
GenBank蛋白编号BC000614
基因名称 HDAC3
Gene ID (NCBI) 8841
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15379
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (-amino groups of lysines located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene.