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验证数据展示

  • WB analysis using 28569-1-AP

    WB analysis using 28569-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28569-1-AP (HCFC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 28569-1-AP

    IHC staining of mouse liver using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HCFC1 antibody included
  • IHC staining of mouse liver using 28569-1-AP

    IHC staining of mouse liver using 28569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HCFC1 antibody included
  • IF Staining of HeLa using 28569-1-AP

    IF Staining of HeLa using 28569-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HCFC1 antibody (28569-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IP experiment of HeLa using 28569-1-AP

    IP experiment of HeLa using 28569-1-AP

    IP result of anti-HCFC1 (IP:28569-1-AP, 4ug; Detection:28569-1-AP 1:20000) with HeLa cells lysate 1600 ug.

HCFC1 Polyclonal antibody

HCFC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 28569-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

HCF C-terminal chain 2, HCF C-terminal chain 1, HCF 1, HCF, CFF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, NIH/3T3 cells, PC-12 cells, C2C12 cells, C6 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse liver tissue, human lung cancer tissue, human urothelial carcinoma tissue, mouse cerebellum tissue, mouse lung tissue, mouse skin tissue, rat cerebellum tissue, rat lung tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HCFC1 Ready-To-Use IHC Kit. HCFC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28569-1-AP targets HCFC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29663

Product name: Recombinant human HCFC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1018-1174 aa of NM_005334

Sequence: CETHETGTTNTATTTVVANLGGHPQPTQVQFVCDRQEAAASLVTSTVGQQNGSVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTNTATTAMSSVGANHQRDARRACAAGTPAVIRISVATGALEAAQGSKSQCQTRQTSAT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 host cell factor C1 (VP16-accessory protein)
别名 HCF C-terminal chain 2, HCF C-terminal chain 1, HCF 1, HCF, CFF
计算分子量 209 kDa
观测分子量300 kDa, 100-150 kDa
GenBank蛋白编号NM_005334
基因名称 HCFC1
Gene ID (NCBI) 3054
RRIDAB_3086066
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51610
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HCFC1 (also known as HCF-1) was discovered in studies of herpes simplex virus (HSV) transcription. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in the control of the cell cycle and transcriptional regulation during herpes simplex virus infection. HCFC1 is a large protein of ∼300 kDa and is proteolytically processed into N- and C-terminal fragments of ∼150 kDa each.(PMID: 21295698 )

实验方案

Product Specific Protocols
WB protocol for HCFC1 antibody 28569-1-APDownload protocol
IHC protocol for HCFC1 antibody 28569-1-APDownload protocol
IF protocol for HCFC1 antibody 28569-1-APDownload protocol
IP protocol for HCFC1 antibody 28569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols