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验证数据展示

  • WB analysis of Jurkat using 10359-1-AP (same clone as 10359-1-PBS)

    WB analysis of Jurkat using 10359-1-AP (same clone as 10359-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 10359-1-AP (same clone as 10359-1-PBS)

    IHC staining of human pancreas cancer using 10359-1-AP (same clone as 10359-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10359-1-AP (HBS1L antibody) at dilution of 1:50 (under 25x lens). This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 10359-1-AP (same clone as 10359-1-PBS)

    IP experiment of mouse brain using 10359-1-AP (same clone as 10359-1-PBS)

    IP result of anti-HBS1L (IP:10359-1-AP, 4ug; Detection:10359-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 10359-1-AP (same clone as 10359-1-PBS)

    WB analysis of Jurkat using 10359-1-AP (same clone as 10359-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

  • WB analysis using 10359-1-AP (same clone as 10359-1-PBS)

    WB analysis using 10359-1-AP (same clone as 10359-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 10359-1-AP (same clone as 10359-1-PBS)

    WB analysis of HEK-293 using 10359-1-AP (same clone as 10359-1-PBS)

    WB result of HBS1L antibody (10359-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells. This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 10359-1-AP (same clone as 10359-1-PBS)

    IF Staining of HepG2 using 10359-1-AP (same clone as 10359-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HBS1L antibody (10359-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HBS1L Polyclonal antibody, PBS Only

HBS1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10359-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EC:3.6.5.-, EF 1a, ERFS, HBS 1, HBS1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10359-1-PBS targets HBS1L in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0388

Product name: Recombinant human HBS1L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 43-262 aa of BC001465

Sequence: SRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMREVLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKPVDSQTSRSESEIVPKVAKMTVSGKKQTMGFEVPGVSSEENGHSFHTPQKGPPIEDAIASSDVLETASKSANPPHTIQASEEQSSTPAPVKKSGKLRQQIDVKAELEKRQGGKQLLN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HBS1-like (S. cerevisiae)
别名 EC:3.6.5.-, EF 1a, ERFS, HBS 1, HBS1
计算分子量 75 kDa
观测分子量 75 kDa
GenBank蛋白编号BC001465
基因名称 HBS1L
Gene ID (NCBI) 10767
RRIDAB_2114730
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y450
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.

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