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验证数据展示

  • FC experiment of K-562 using CL750-16216

    FC experiment of K-562 using CL750-16216

    1X10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite® Plus 750 Anti-Human HBB (CL750-16216) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of human appendicitis using CL750-16216

    IF Staining of human appendicitis using CL750-16216

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 750 HBB antibody (CL750-16216) at dilution of 1:200.

CoraLite® Plus 750-conjugated HBB Polyclonal antibody

HBB Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL750-16216

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P, FC (Intra)

别名

Spinorphin, LVV-hemorphin-7, Hemoglobin subunit beta, Hemoglobin beta chain, Beta-globin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman appendicitis tissue
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy HBB Ready-To-Use IHC Kit. HBB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16216-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-16216 targets HBB in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 HBB fusion protein Ag9216 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemoglobin, beta
别名 Spinorphin, LVV-hemorphin-7, Hemoglobin subunit beta, Hemoglobin beta chain, Beta-globin
计算分子量 147 aa, 16 kDa
观测分子量 13-16 kDa
GenBank蛋白编号BC007075
基因名称 HBB
Gene ID (NCBI) 3043
RRIDAB_3673690
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP68871
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.

实验方案

Product Specific Protocols
IF protocol for CL Plus 750 HBB antibody CL750-16216Download protocol
Standard Protocols
Click here to view our Standard Protocols