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KIM-1/HAVCR1 Polyclonal antibody

KIM-1/HAVCR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 30948-1-AP

Print datasheet

Synonyms

CD365, Havcr1, KIM 1, KIM1, TIM-1, TIMD-1

验证数据展示

  • WB analysis using 30948-1-AP

    WB analysis using 30948-1-AP

    Mouse kidney tissue was subjected to SDS PAGE followed by western blot with 30948-1-AP (KIM-1/HAVCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 30948-1-AP

    IHC staining of rat kidney using 30948-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30948-1-AP (KIM-1/HAVCR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 30948-1-AP

    IHC staining of rat kidney using 30948-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30948-1-AP (KIM-1/HAVCR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30948-1-AP targets KIM-1/HAVCR1 in WB, IHC, ELISA applications and shows reactivity with Mouse, Rat samples.

Tested Applications WB, IHC, ELISA
Tested Reactivity Mouse, Rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hepatitis A virus cellular receptor 1
Synonyms CD365, Havcr1, KIM 1, KIM1, TIM-1, TIMD-1
Calculated Molecular Weight34 kDa
Observed Molecular Weight72 kDa
GenBank Accession NumberNM_173149.2
Gene Symbol Havcr1
Gene ID (NCBI) 286934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO54947
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kidney injury molecule 1 (KIM-1), also known as Hepatitis A virus cellular receptor 1 (HAVCR1), CD365, or T-cell immunoglobulin and mucin domain 1 (TIM-1), is a class I integral membrane glycoprotein, with an ectodomain containing Ig-like domain and a mucin domain. KIM-1 acts as a membrane receptor for hepatitis A virus (HAV) (PMID: 9658108; 8861957). KIM-1 provides a costimulatory signal for T cell activation and inhibits the development of peripheral tolerance (PMID: 16284246; 15793575). KIM-1 may be involved in the regulation of asthma and allergic diseases (PMID: 14534576). It has been reported that KIM-1 is shed into urine after acute kidney damage and is a marker of renal tubular injury (PMID: 14600030).

实验方案

Product Specific Protocols
WB protocol for KIM-1/HAVCR1 antibody 30948-1-APDownload protocol
IHC protocol for KIM-1/HAVCR1 antibody 30948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols