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验证数据展示

  • IHC staining of human pancreas cancer using 11094-2-AP (same clone as 11094-2-PBS)

    IHC staining of human pancreas cancer using 11094-2-AP (same clone as 11094-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HAUS1 antibody included
  • IHC staining of human pancreas cancer using 11094-2-AP (same clone as 11094-2-PBS)

    IHC staining of human pancreas cancer using 11094-2-AP (same clone as 11094-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HAUS1 antibody included
  • WB analysis of human skeletal muscle using 11094-2-AP (same clone as 11094-2-PBS)

    WB analysis of human skeletal muscle using 11094-2-AP (same clone as 11094-2-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11094-2-AP (same clone as 11094-2-PBS)

    IP experiment of HeLa using 11094-2-AP (same clone as 11094-2-PBS)

    IP result of anti-HAUS1 (IP:11094-2-AP, 4ug; Detection:11094-2-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

HAUS1 Polyclonal antibody, PBS Only

HAUS1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11094-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

CCDC5, Coiled-coil domain-containing protein 5, Enhancer of invasion cluster, Enhancer of invasion-cluster, HAUS augmin-like complex subunit 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HAUS1 Ready-To-Use IHC Kit. HAUS1 primary antibody included.

产品信息

11094-2-PBS targets HAUS1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1569

Product name: Recombinant human CCDC5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-278 aa of BC014003

Sequence: MEPQEERETQVAAWLKKIFGDHPIPQYEVNPRTTEILHHLSERNRVRDRDVYLVIEDLKQKASEYESEAKYLQDLLMESVNFSPANLSSTGSRYLNALVDSAVALETKDTSLASFIPAVNDLTSDLFRTKSKSEEIKIELEKLEKNLTATLVLEKCLQEDVKKAELHLSTERAKVDNRRQNMDFLKAKSEEFRFGIKAAEEQLSARGMDASLSHQSLVALSEKLARLKQQTIPLKKKLESYLDLMPNPSLAQVKIEEAKRELDSIEAELTRRVDMMEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 5 (spindle associated)
别名 CCDC5, Coiled-coil domain-containing protein 5, Enhancer of invasion cluster, Enhancer of invasion-cluster, HAUS augmin-like complex subunit 1
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC014003
基因名称 HAUS1
Gene ID (NCBI) 115106
RRIDAB_614986
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96CS2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HAUS1 is a subunit of the human augmin complex that is involved in microtubule generation and mitotic spindle formation (PMID:27036161). In addition, HAUS1 may play a major role in centrosome integrity and completion of cytokinesis. The depletion of HAUS1 in cells decreases the G2/M cell compartment and induces apoptosis. High HAUS1 expression was associated with several tumour-infiltrating immune cells such as Th2 cells, macrophages, and activated dendritic cells (PMID:35865089). 11094-2-AP can detect the 32 kDa band, which is consistent with the calculated MW.

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