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HAUS1 Polyclonal antibody

HAUS1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11094-2-AP

Print datasheet

Synonyms

CCDC5, Enhancer of invasion cluster, HAUS1, HEI C, HEIC, HsT1461

验证数据展示

  • IHC staining of human pancreas cancer using 11094-2-AP

    IHC staining of human pancreas cancer using 11094-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 11094-2-AP

    IHC staining of human pancreas cancer using 11094-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human skeletal muscle using 11094-2-AP

    WB analysis of human skeletal muscle using 11094-2-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 11094-2-AP

    IP experiment of HeLa using 11094-2-AP

    IP result of anti-HAUS1 (IP:11094-2-AP, 4ug; Detection:11094-2-AP 1:500) with HeLa cells lysate 1200ug.

经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, HL-60 cells, HeLa cells, MOLT-4 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11094-2-AP targets HAUS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen HAUS1 fusion protein Ag1569 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 5 (spindle associated)
Synonyms CCDC5, Enhancer of invasion cluster, HAUS1, HEI C, HEIC, HsT1461
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC014003
Gene Symbol CCDC5
Gene ID (NCBI) 115106
RRIDAB_614986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CS2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HAUS1 is a subunit of the human augmin complex that is involved in microtubule generation and mitotic spindle formation (PMID:27036161). In addition, HAUS1 may play a major role in centrosome integrity and completion of cytokinesis. The depletion of HAUS1 in cells decreases the G2/M cell compartment and induces apoptosis. High HAUS1 expression was associated with several tumour-infiltrating immune cells such as Th2 cells, macrophages, and activated dendritic cells (PMID:35865089). 11094-2-AP can detect the 32 kDa band, which is consistent with the calculated MW.

实验方案

Product Specific Protocols
WB protocol for HAUS1 antibody 11094-2-APDownload protocol
IHC protocol for HAUS1 antibody 11094-2-APDownload protocol
IP protocol for HAUS1 antibody 11094-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article
mouseWB

Nucleic Acids Res

An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity

Authors - Dan Li
View Article