- Featured Product
- KD/KO Validated
HAT1 Polyclonal antibody
HAT1 Polyclonal Antibody for IF/ICC,IF-P, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC,IF-P, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, MDA-MB-231 cells, NIH/3T3 cells, C6 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse ovary tissue, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HAT1 Ready-To-Use IHC Kit. HAT1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11432-1-AP targets HAT1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC,IF-P, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | HAT1 fusion protein Ag1952 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | histone acetyltransferase 1 |
| Synonyms | HAT1, histone acetyltransferase 1, KAT1 |
| Calculated Molecular Weight | 419 aa, 50 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC018682 |
| Gene Symbol | HAT1 |
| Gene ID (NCBI) | 8520 |
| RRID | AB_2116435 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14929 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HAT1 antibody 11432-1-AP | Download protocol |
| IHC protocol for HAT1 antibody 11432-1-AP | Download protocol |
| IF protocol for HAT1 antibody 11432-1-AP | Download protocol |
| IP protocol for HAT1 antibody 11432-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.
| ||
Mol Cell Proteomics Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). | ||
Alcohol Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism. | ||
Biochem Biophys Res Commun Identification and characterization of an endoplasmic reticulum localization motif. |
