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验证数据展示

  • WB analysis of fetal human brain using 25133-1-AP (same clone as 25133-1-PBS)

    WB analysis of fetal human brain using 25133-1-AP (same clone as 25133-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25133-1-AP (HAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 25133-1-AP (same clone as 25133-1-PBS)

    IHC staining of mouse brain using 25133-1-AP (same clone as 25133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25133-1-AP (HAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HAP1 antibody included
  • IHC staining of mouse brain using 25133-1-AP (same clone as 25133-1-PBS)

    IHC staining of mouse brain using 25133-1-AP (same clone as 25133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25133-1-AP (HAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HAP1 antibody included
  • IF Staining of SH-SY5Y using 25133-1-AP (same clone as 25133-1-PBS)

    IF Staining of SH-SY5Y using 25133-1-AP (same clone as 25133-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 25133-1-AP (HAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

HAP1 Polyclonal antibody, PBS Only

HAP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25133-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HAP1 Ready-To-Use IHC Kit. HAP1 primary antibody included.

产品信息

25133-1-PBS targets HAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag17705

Product name: Recombinant human HAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 331-671 aa of BC156728

Sequence: LREEASQLDTLEDEEQMLILECVEQFSEASQQMAELSEVLVLRLENYERQQQEVARLQAQVLKLQQRCRMYGAETEKLQKQLASEKEIQMQLQEESVWVGSQLQDLREKYMDCGGMLIEMQEEVKTLRQQPPVSTGSATHYPYSVPLETLPGFQETLAEELRTSLRRMISDPVYFMERNYEMPRGDTSSLRYDFRYSEDREQVRGFEAEEGLMLAADIMRGEDFTPAEEFVPQEELGAAKKVPAEEGVMEEAELVSEETEGWEEVELELDEATRMNVVTSALEASGLGPSHLDMNYVLQQLANWQDAHYRRQLRWKMLQKGECPHGALPAASRTSCRSSCR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 huntingtin-associated protein 1
别名
计算分子量 671 aa, 76 kDa
观测分子量 75-80 kDa
GenBank蛋白编号BC156728
基因名称 HAP1
Gene ID (NCBI) 9001
RRIDAB_2879915
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54257
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HAP1 (Huntingtin-associated protein 1) was originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT. HTT is expressed ubiquitously, while HAP1 is expressed predominantly in the central nervous system (CNS). Both HTT and HAP1 participate in intracellular trafficking. HAP1 is involved in the vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation (PMID: 19262167).

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