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验证数据展示

  • WB analysis of human placenta using 67031-1-Ig (same clone as 67031-1-PBS)

    WB analysis of human placenta using 67031-1-Ig (same clone as 67031-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67031-1-Ig (HAI-1 antibody) at dilution of 1:900 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67031-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67031-1-Ig (same clone as 67031-1-PBS)

    IF Staining of MCF-7 using 67031-1-Ig (same clone as 67031-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using HAI-1 antibody (67031-1-Ig, Clone: 2H2D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67031-1-PBS in a different storage buffer formulation.

SPINT1/HAI-1 Monoclonal antibody, PBS Only

SPINT1/HAI-1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67031-1-PBS

产品说明书

CloneNo. 2H2D8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

HAI 1, HAI-1, SPINT1, 2H2D8, HAI

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67031-1-PBS targets SPINT1/HAI-1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 SPINT1/HAI-1 fusion protein Ag26733 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serine peptidase inhibitor, Kunitz type 1
别名 HAI 1, HAI-1, SPINT1, 2H2D8, HAI
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC004140
基因名称 HAI-1
Gene ID (NCBI) 6692
RRIDAB_2882346
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43278
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is also named as SPINT1 and Kunitz-type protease inhibitor 1. HAI-1 is critical to control matriptase proteolytic activity (PMID: 25310042). HAI-1 inhibits matriptase activity through forming complex with activated matriptase (PMID: 30370662). matriptase, HAI‐1 also binds to and inhibits a variety of serine proteases including of hepsin, plasmin, prostasin, TMPRSS13 and HAT. HAI-1 as a cell-autonomous tumor suppressor that negatively regulates the HGF pathway (PMID: 25925948). HAI-1 is inhibitor of HGFAC (PMID: 9045658). HAI-1 inhibits serine protease activity of ST14/matriptase in vitro (PMID: 28710277). HAI-1 inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PMID: 20977675). HAI-1 expression is reduced in many cancer types, which is implicated in the progression of cancer and is associated with a worse prognosis in cancer patients (PMID: 30370662).