验证数据展示
经过测试的应用
Positive WB detected in | human placenta tissue |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
ChIP | See 1 publications below |
产品信息
67031-1-Ig targets SPINT1/HAI-1 in WB, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, ChIP |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | SPINT1/HAI-1 fusion protein Ag26733 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | serine peptidase inhibitor, Kunitz type 1 |
别名 | HAI 1, HAI-1, SPINT1, 2H2D8, HAI |
计算分子量 | 58 kDa |
观测分子量 | 58 kDa |
GenBank蛋白编号 | BC004140 |
基因名称 | HAI-1 |
Gene ID (NCBI) | 6692 |
RRID | AB_2882346 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | O43278 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is also named as SPINT1 and Kunitz-type protease inhibitor 1. HAI-1 is critical to control matriptase proteolytic activity (PMID: 25310042). HAI-1 inhibits matriptase activity through forming complex with activated matriptase (PMID: 30370662). matriptase, HAI‐1 also binds to and inhibits a variety of serine proteases including of hepsin, plasmin, prostasin, TMPRSS13 and HAT. HAI-1 as a cell-autonomous tumor suppressor that negatively regulates the HGF pathway (PMID: 25925948). HAI-1 is inhibitor of HGFAC (PMID: 9045658). HAI-1 inhibits serine protease activity of ST14/matriptase in vitro (PMID: 28710277). HAI-1 inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PMID: 20977675). HAI-1 expression is reduced in many cancer types, which is implicated in the progression of cancer and is associated with a worse prognosis in cancer patients (PMID: 30370662).
实验方案
Product Specific Protocols | |
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WB protocol for SPINT1/HAI-1 antibody 67031-1-Ig | Download protocol |
IF protocol for SPINT1/HAI-1 antibody 67031-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |