SPINT1/HAI-1 Monoclonal antibody

SPINT1/HAI-1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67031-1-Ig

产品说明书

CloneNo. 2H2D8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA and More (1)

HAI 1, HAI-1, SPINT1, 2H2D8, HAI

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存


经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67031-1-Ig targets SPINT1/HAI-1 in WB, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, ChIP
经测试反应性 human
文献引用反应性human
免疫原 SPINT1/HAI-1 fusion protein Ag26733 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serine peptidase inhibitor, Kunitz type 1
别名 HAI 1, HAI-1, SPINT1, 2H2D8, HAI
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC004140
基因名称 HAI-1
Gene ID (NCBI) 6692
RRIDAB_2882346
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43278
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is also named as SPINT1 and Kunitz-type protease inhibitor 1. HAI-1 is critical to control matriptase proteolytic activity (PMID: 25310042). HAI-1 inhibits matriptase activity through forming complex with activated matriptase (PMID: 30370662). matriptase, HAI‐1 also binds to and inhibits a variety of serine proteases including of hepsin, plasmin, prostasin, TMPRSS13 and HAT. HAI-1 as a cell-autonomous tumor suppressor that negatively regulates the HGF pathway (PMID: 25925948). HAI-1 is inhibitor of HGFAC (PMID: 9045658). HAI-1 inhibits serine protease activity of ST14/matriptase in vitro (PMID: 28710277). HAI-1 inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PMID: 20977675). HAI-1 expression is reduced in many cancer types, which is implicated in the progression of cancer and is associated with a worse prognosis in cancer patients (PMID: 30370662).

实验方案

Product Specific Protocols
WB protocol for SPINT1/HAI-1 antibody 67031-1-IgDownload protocol
IF protocol for SPINT1/HAI-1 antibody 67031-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,ChIP

J Cell Biol

Matriptase drives dissemination of ovarian cancer spheroids by a PAR-2/PI3K/Akt/MMP9 signaling axis

Authors - Nisha R Pawar
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