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验证数据展示

  • IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60250-1-Ig (HADHA antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK293 cells using 60250-1-Ig (same clone as 60250-1-PBS)

    WB analysis of HEK293 cells using 60250-1-Ig (same clone as 60250-1-PBS)

    WB result of HADHA specific antibody (60250-1-Ig, 1:4000) with si-control and si-HADHA transfected HEK293 cells. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • WB analysis using 60250-1-Ig (same clone as 60250-1-PBS)

    WB analysis using 60250-1-Ig (same clone as 60250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 60250-1-Ig (same clone as 60250-1-PBS)

    IHC staining of mouse heart using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60250-1-Ig (HADHA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HADHA antibody included
  • Cytometric bead array standard curve of MP50412-1

    Cytometric bead array standard curve of MP50412-1

    Cytometric bead array standard curve of MP50412-1, HADHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60250-1-PBS. Detection antibody: 60250-2-PBS. Standard:Ag1211. Range: 1.563-100 ng/mL.

  • Affinity and Kinetic Characterization of 60250-1-Ig

    Affinity and Kinetic Characterization of 60250-1-Ig

    Biolayer interferometry (BLl) kinetic assay of 60250-1-Ig against Human HADHA was performed. The affinity constant is 17.3 nM.

  • Featured Product
  • KD/KO验证

HADHA Monoclonal antibody, PBS Only (Capture)

HADHA Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 60250-1-PBS

产品说明书

CloneNo. 3E9B1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

3E9B1, 78 kDa gastrin binding protein, 78 kDa gastrin-binding protein, EC:2.3.1.-, ECHA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HADHA Ready-To-Use IHC Kit. HADHA primary antibody included.

产品信息

60250-1-PBS targets HADHA as part of a matched antibody pair:

MP50412-1: 60250-1-PBS capture and 60250-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1211

Product name: Recombinant human HADHA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 339-763 aa of BC009235

Sequence: LMGLYHGQVLCKKNKFGAPQKDVKHLAILGAGLMGAGIAQVSVDKGLKTILKDATLTALDRGQQQVFKGLNDKVKKKALTSFERDSIFSNLTGQLDYQGFEKADMVIEAVFEDLSLKHRVLKEVEAVIPDHCIFASNTSALPISEIAAVSKRPEKVIGMHYFSPVDKMQLLEIITTEKTSKDTSASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDSLTTSFGFPVGAATLVDEVGVDVAKHVAEDLGKVFGERFGGGNPELLTQMVSKGFLGRKSGKGFYIYQEGVKRKDLNSDMDSILASLKLPPKSEVSSDEDIQFRLVTRFVNEAVMCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKIVDRLKKYEAAYGKQFTPCQLLADHANSPNKKFYQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
别名 3E9B1, 78 kDa gastrin binding protein, 78 kDa gastrin-binding protein, EC:2.3.1.-, ECHA
计算分子量 83 kDa
观测分子量 79 kDa
GenBank蛋白编号BC009235
基因名称 HADHA
Gene ID (NCBI) 3030
RRIDAB_2881371
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP40939
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).

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