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验证数据展示

  • WB analysis using 81290-1-RR

    WB analysis using 81290-1-RR

    HA-tag recombinant protein, non-transfected HEK-293 cells, transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81290-1-RR (HA Tag antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IP experiment of Transfected HEK-293T using 81290-1-RR

    IP experiment of Transfected HEK-293T using 81290-1-RR

    IP result of anti-HA Tag (IP:81290-1-RR, 4ug; Detection:81290-1-RR 1:10000) with Transfected HEK-293T cells lysate 400 ug.

  • IF Staining of Transfected HEK-293 using 81290-1-RR

    IF Staining of Transfected HEK-293 using 81290-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (81290-1-RR, Clone: 6I21 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Transfected HEK-293 using 81290-1-RR

    FC experiment of Transfected HEK-293 using 81290-1-RR

    1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug HA Tag Recombinant antibody (81290-1-RR, Clone:6I21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of Transfected HEK-293 using 81290-1-RR

    IF Staining of Transfected HEK-293 using 81290-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using HA Tag antibody (81290-1-RR, Clone: 6I21 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 81290-1-RR

    Affinity and Kinetic Characterization of 81290-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81290-1-RR against a protein with HA tag were performed. The affinity constant is 0.766 nM.

HA Tag Recombinant monoclonal antibody

HA Tag Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 81290-1-RR

产品说明书

CloneNo. 6I21
引用文献(129)

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein and More (4)

应用

WB, IF/ICC, FC (Intra), IP, ELISA and More (3)

别名

HA

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTransfected HEK-293 cells
Positive IP detected inTransfected HEK-293T cells
Positive IF/ICC detected inTransfected HEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81290-1-RR targets HA Tag in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 recombinant protein
文献引用反应性human, mouse, chicken, hamster
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 HA Tag
别名 HA
计算分子量 1 kDa
基因名称
Gene ID (NCBI)
RRIDAB_2935602
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDHATAG
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

实验方案

Product Specific Protocols
FC protocol for HA Tag antibody 81290-1-RRDownload protocol
IF protocol for HA Tag antibody 81290-1-RRDownload protocol
IP protocol for HA Tag antibody 81290-1-RRDownload protocol
WB protocol for HA Tag antibody 81290-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IP

Signal Transduct Target Ther

DNA/RNA-binding protein KIN17 supports esophageal cancer progression via resolving noncanonical STING activation induced by R-loop.

Authors - Zichao Wei
View Article
WB

Nat Metab

Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination

Authors - Yuxia Sun
View Article
IF

Nat Cell Biol

Manganese regulation of COPII condensation controls circulating lipid homeostasis

Authors - Xiao Wang
View Article
WB

Nat Cell Biol

Defining heterogeneity in core regulatory circuitry reveals HOXB3 condensation as a potential target in glioblastoma.

Authors - Chuanxia Zhang
View Article

Drug Resist Updat

Lactate-driven H3K18 lactylation promotes cisplatin resistance in bladder cancer via HNRNPF-Parkin mediated mitophagy.

Authors - Henchui Zhang
View Article
WB,IP

Nat Commun

Hydroxylation of the HIV-1 antisense protein promotes immune evasion of HIV-1 via modulation of TBK1.

Authors - Xinguang Lin
View Article
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