验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse liver tissue, HeLa cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human hepatocirrhosis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15255-1-AP targets H6PD in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | H6PD fusion protein Ag7109 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) |
| 别名 | 6PGL, G6PDH, GDH, H6PD |
| 计算分子量 | 89 kDa |
| 观测分子量 | 89-95 kDa |
| GenBank蛋白编号 | BC081559 |
| 基因名称 | H6PD |
| Gene ID (NCBI) | 9563 |
| RRID | AB_10642699 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95479 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hexose-6-phosphate dehydrogenase (H6PD) is also named as GDH,6PGL. It is the main NADPH generating enzyme in the lumen of the endoplasmic reticulum. H6PD is regarded as an ancillary enzyme in prereceptorial glucocorticoid activation and probably acts as a nutrient sensor and as a prosurvival factor(PMID:21620971). H6PD is a glycoprotein and has been shown tissue differences in glycosylation status might explain both the difference in migration on SDS-PAGE gels as well as in activity. Defects in H6PD are a cause of cortisone reductase deficiency (CRD). This antibody is specific to H6PD.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for H6PD antibody 15255-1-AP | Download protocol |
| IHC protocol for H6PD antibody 15255-1-AP | Download protocol |
| IP protocol for H6PD antibody 15255-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Basic Res Cardiol Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart. | ||
iScience A fingerprint of 2-[18F]FDG radiometabolites - How tissue-specific metabolism beyond 2-[18F]FDG-6-P could affect tracer accumulation
| ||
