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验证数据展示

  • IF Staining of human tonsillitis using CL488-67272

    IF Staining of human tonsillitis using CL488-67272

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 Granzyme K antibody (CL488-67272, Clone: 1C3A4 ) at dilution of 1:200. DAPI (blue).

  • IF Staining of NIH/3T3 using CL488-67272

    IF Staining of NIH/3T3 using CL488-67272

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 Granzyme K antibody (CL488-67272, Clone: 1C3A4 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Granzyme K Monoclonal antibody

Granzyme K Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-67272

产品说明书

CloneNo. 1C3A4

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, IF-P

别名

GZMK, 1C3A4, EC:3.4.21.-, Fragmentin 3, Fragmentin-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 67272-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67272 targets Granzyme K in IF/ICC, IF-P applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human
免疫原

CatNo: Ag27297

Product name: Recombinant human GZMK protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 85-215 aa of BC035802

Sequence: HSLSKNEASKQTLEIKKFIPFSRVTSDPQSNDIMLVKLQTAAKLNKHVKMLHIRSKTSLRSGTKCKVTGWGATDPDSLRPSDTLREVTVTVLSRKLCNSQSYYNGDPFITKDMVCAGDAKGQKDSCKGDSG

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 granzyme K (granzyme 3; NK tryptase II)
别名 GZMK, 1C3A4, EC:3.4.21.-, Fragmentin 3, Fragmentin-3
计算分子量 29 kDa
GenBank蛋白编号BC035802
基因名称 GZMK
Gene ID (NCBI) 3003
RRIDAB_2919442
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49863
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Granzyme K antibody CL488-67272Download protocol
Standard Protocols
Click here to view our Standard Protocols