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CoraLite® Plus 488-conjugated Granzyme K Monoclonal antibody

Granzyme K Monoclonal Antibody for FC (Intra), IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra), IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C3A4

Cat No : CL488-67272

Print datasheet

Synonyms

Fragmentin 3, Granzyme 3, Granzyme K, GZMK, NK Tryp 2, NK tryptase 2, TRYP2

验证数据展示

  • IF Staining of human tonsillitis using CL488-67272

    IF Staining of human tonsillitis using CL488-67272

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 Granzyme K antibody (CL488-67272, Clone: 1C3A4 ) at dilution of 1:200. DAPI (blue).

  • FC experiment of NK92 using CL488-67272

    FC experiment of NK92 using CL488-67272

    1X10^6 NK92 were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Granzyme K (CL488-67272, Clone:1C3A4) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inNK92, NK92 cells
For other applications, we recommend the unconjugated version of this antibody, 67272-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67272 targets Granzyme K in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity Human
Immunogen Granzyme K fusion protein Ag27297 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name granzyme K (granzyme 3; NK tryptase II)
Synonyms Fragmentin 3, Granzyme 3, Granzyme K, GZMK, NK Tryp 2, NK tryptase 2, TRYP2
Calculated Molecular Weight 29 kDa
GenBank Accession NumberBC035802
Gene Symbol GZMK
Gene ID (NCBI) 3003
RRIDAB_2919442
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49863
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Granzyme K antibody CL488-67272Download protocol
FC protocol for CL Plus 488 Granzyme K antibody CL488-67272Download protocol
Standard Protocols
Click here to view our Standard Protocols