验证数据展示
经过测试的应用
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | NIH/3T3 cells |
For other applications, we recommend the unconjugated version of this antibody, 67272-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67272 targets Granzyme K in IF/ICC, IF-P applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, IF-P Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag27297 Product name: Recombinant human GZMK protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 85-215 aa of BC035802 Sequence: HSLSKNEASKQTLEIKKFIPFSRVTSDPQSNDIMLVKLQTAAKLNKHVKMLHIRSKTSLRSGTKCKVTGWGATDPDSLRPSDTLREVTVTVLSRKLCNSQSYYNGDPFITKDMVCAGDAKGQKDSCKGDSG 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | granzyme K (granzyme 3; NK tryptase II) |
| 别名 | GZMK, 1C3A4, EC:3.4.21.-, Fragmentin 3, Fragmentin-3 |
| 计算分子量 | 29 kDa |
| GenBank蛋白编号 | BC035802 |
| 基因名称 | GZMK |
| Gene ID (NCBI) | 3003 |
| RRID | AB_2919442 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P49863 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Granzyme K antibody CL488-67272 | Download protocol |
| IF protocol for CL Plus 488 Granzyme K antibody CL488-67272 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
