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验证数据展示

  • FC experiment of mouse bone marrow using 98269-1-RR (same clone as 98269-1-PBS)

    FC experiment of mouse bone marrow using 98269-1-RR (same clone as 98269-1-PBS)

    1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody (98269-1-RR, Clone: 242129D10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD45 (30-F11) (APC-65087, Clone: 30-F11). Cells were not fixed. This data was developed using the same antibody clone with 98269-1-PBS in a different storage buffer formulation.

  • FC experiment of mouse bone marrow using 98269-1-RR (same clone as 98269-1-PBS)

    FC experiment of mouse bone marrow using 98269-1-RR (same clone as 98269-1-PBS)

    1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody (98269-1-RR, Clone: 242129D10) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98269-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98269-1-RR

    Affinity and Kinetic Characterization of 98269-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98269-1-RR against Mouse Glycophorin A/CD235a were performed. The affinity constant is 19.4 nM.

Anti-Mouse Glycophorin A/CD235a Rabbit Recombinant Antibody, PBS Only

Glycophorin A/CD235a Uni-rAbTM Recombinant Antibody for FC
Cat No. 98269-1-PBS

产品说明书

CloneNo. 242129D10

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

glycophorin A, Gypa, GPA, Glycophorin-A, CD235a

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98269-1-PBS targets Glycophorin A/CD235a in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glycophorin A
别名 glycophorin A, Gypa, GPA, Glycophorin-A, CD235a
计算分子量18kDa
GenBank蛋白编号NM_010369.3
基因名称 Gypa
Gene ID (NCBI) 14934
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP14220
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycophorin A (GYPA), also known as CD235a, is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)