验证数据展示
产品信息
CL555-66323 targets Glutamine Synthetase in IF applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IF Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | Glutamine Synthetase fusion protein Ag6309 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glutamate-ammonia ligase (glutamine synthetase) |
| 别名 | GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59 |
| 计算分子量 | 374 aa, 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC011700 |
| 基因名称 | Glutamine Synthetase |
| Gene ID (NCBI) | 2752 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P15104 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLUL(Glutamine synthetase) is also named as GS, GLNS, which belongs to the glutamine synthetase family. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for the proliferation of fetal skin fibroblasts(PMID:18662667). Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 Glutamine Synthetase antibody CL555-66323 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |