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验证数据展示

  • IF Staining of A549 using CL594-24050

    IF Staining of A549 using CL594-24050

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 Glucocorticoid receptor antibody (CL594-24050) at dilution of 1:200.

  • FC experiment of HeLa using CL594-24050

    FC experiment of HeLa using CL594-24050

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Glucocorticoid receptor (CL594-24050) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Glucocorticoid receptor Polyclonal antibody

Glucocorticoid receptor Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-24050

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

NR3C1, GCCR, GCR, GR, GRL

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24050-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-24050 targets Glucocorticoid receptor in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21146

Product name: Recombinant human Glucocorticoid receptor protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC015610

Sequence: MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASSPSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGFPQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDVSSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDENCLLSPLAGEDDSFLLEGNSNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEKEDFIELCTPGVIKQEKLGTVYCQASFPGANIIGNKMSAISVHGVSTSGGQMYHYDMNTASLSQQQDQKPI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
别名 NR3C1, GCCR, GCR, GR, GRL
计算分子量 86 kDa
观测分子量 94-97 kDa
GenBank蛋白编号BC015610
基因名称 Glucocorticoid receptor
Gene ID (NCBI) 2908
RRIDAB_2919872
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04150
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].

实验方案

Product Specific Protocols
FC protocol for CL594 Glucocorticoid receptor antibody CL594-24050Download protocol
IF protocol for CL594 Glucocorticoid receptor antibody CL594-24050Download protocol
Standard Protocols
Click here to view our Standard Protocols
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