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验证数据展示

  • WB analysis using 82619-4-RR (same clone as 82619-4-PBS)

    WB analysis using 82619-4-RR (same clone as 82619-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    IP experiment of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    IP result of anti-Glucocorticoid receptor (IP:82619-4-RR, 4ug; Detection:82619-4-RR 1:8000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:350 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR3C1 antibody included
  • IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:350 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR3C1 antibody included
  • IF Staining of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    IF Staining of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (82619-4-RR, Clone: 2F22 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82619-4-RR

    Affinity and Kinetic Characterization of 82619-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82619-4-RR against Human Glucocorticoid receptor were performed. The affinity constant is 0.693 nM.

Glucocorticoid receptor Recombinant monoclonal antibody, PBS Only

Glucocorticoid receptor Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82619-4-PBS

产品说明书

CloneNo. 2F22

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

NR3C1, GRL, GR, GCR, GCCR

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.

产品信息

82619-4-PBS targets Glucocorticoid receptor in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21146

Product name: Recombinant human Glucocorticoid receptor protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC015610

Sequence: MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASSPSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGFPQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDVSSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDENCLLSPLAGEDDSFLLEGNSNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEKEDFIELCTPGVIKQEKLGTVYCQASFPGANIIGNKMSAISVHGVSTSGGQMYHYDMNTASLSQQQDQKPI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
别名 NR3C1, GRL, GR, GCR, GCCR
计算分子量 86 kDa
观测分子量 94-97 kDa
GenBank蛋白编号BC015610
基因名称 Glucocorticoid receptor
Gene ID (NCBI) 2908
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04150
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

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